Virus-Dependent Immune Conditioning of Tissue Microenvironments

Tissue-Dependent Tumor Microenvironments and Their Impact on Immunotherapy Responses

Recent advances in cancer immunology have led to a better understanding of the role of the tumor microenvironment (TME) in tumor initiation, progression, and metastasis. Tumors can occur at many locations within the body and coevolution between malignant tumor cells and non-malignant cells sculpts the TME at these sites. It has become increasingly clear that there are specific differences of th...

Tissue resident memory T cells create genetically distinct immune microenvironments within melanoma metastasis

Blockade of inhibitory immune checkpoints (ICPs) improves survival in melanoma patients. Interestingly, the expression of these ICPs in most tumor tissues including melanoma is restricted to only a subset of tumor-infiltrating immune cells (TIICs). Here, we utilize single cell mass cytometry (CyTOF), functional profiling and TCR sequencing of freshly isolated cells to evaluate the phenotypic, f...

Tumor Cell Migration Through Tissue Microenvironments

Three-dimensional (3-D) molecular contacts, contributed by the basement membrane, ECM, and adjacent cells, provide an architectural context within which a normal cell functions. Adhesive structures are physical connections between the cytoskeleton (CSK) and the ECM (an extracellularmatrix connection) or between the CSK and the CSK of other cells (a cell-cell connection). There are several adhes...

Diffusion dependent cell behavior in microenvironments.

Understanding the interaction between soluble factors and cells in the cellular microenvironment is critical to understanding a wide range of diseases. Microchannel culture systems provide a tool for separating diffusion and convection based transport making possible controlled studies of the effects of soluble factors in the cellular microenvironment. In this paper we compare the proliferation...

Adaptation of Horsesickness Virus to Tissue Culture